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KMID : 0359319730130010023
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Korean Journal of Veterinary Research
1973 Volume.13 No. 1 p.23 ~ p.30
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Studies on Separation , Detection and Quantitation of Estriol , Estrone , Estradiol - 17 β in Urine of Dairy Cows by Paper , Thin Layer and Column Chromatography
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Abstract
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Thin layer, paper and column chromatography were compared for the separation, detection and quantitation of three kinds of estrogen in urine of dairy cows.
While thin layer chromatography utilizing silica gel was better for the detection of estrogens, column chromatography using celite 545 was preferable.
Spectrophotometry was compared with fluorometry for determination of estrone, estradiol-17 β and estriol eluted by paper chromatography and column chromatography.
Optical density of three standard estrogens showed almost same curve at maximum absorption wave length of 230 and 282mμ. However, the former showed a higher peak.
In fluorometry, the fluorescencefintensity of estrone and estradiol-17 β were rather strong, when the estrogens were dissolved in sulfuric acid, and showed higher sensitivity than that of the spectrophotometry. However, in the case of estriol was exceptional.
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